Differentiation trajectories and biofunctions of symbiotic and un-symbiotic fate cells in root nodules of Medicago truncatula

The root nodule is a complex symbiotic nitrogen fixation factory, in which cells are highly heterogeneous. However, the differentiation trajectories and interconnection of remain largely unknown. In this study, we set up modified protocol for protoplast preparation performed single-cell RNA sequencing profiling indeterminate Medicago truncatula nodule. We designated 13 cell clusters with specific expression patterns 14-day post inoculation nodules constructed spatial functional cellular map based on experimental data bioinformatic analyses. Pseudotime analysis further revealed that two groups apical meristematic develop into un-symbiotic fate along their particular trajectories. Biofunction each cluster particularity interrelation, especially un-infected zone also involved assimilation by undertaking asparagine synthesis. Collectively, our offer an important resource investigating mechanism organogenesis fixation.